The Rat LP (a) Lipoprotein (a) CLIA Kit is a cutting-edge assay designed for the precise measurement of LP (a) levels in rat serum samples. This kit offers exceptional sensitivity and accuracy, guaranteeing consistent and trustworthy results that are invaluable for a variety of research purposes.Lipoprotein (a) is a unique lipid particle that is associated with an increased risk of cardiovascular disease. Monitoring LP (a) levels in rat models can provide crucial insights into the mechanisms underlying atherosclerosis and other cardiovascular conditions, making this kit an indispensable tool for researchers studying these areas.
With its advanced technology and comprehensive features, the Rat LP (a) Lipoprotein (a) CLIA Kit is a must-have for any laboratory conducting research on lipid metabolism and cardiovascular health in rat models. Trust in this high-quality assay kit to deliver reliable data that will drive groundbreaking discoveries in the field.
Product Name:
Rat Lp-a (Lipoprotein a) CLIA Kit
SKU:
RTES00363
Target:
Rat Lp-a (Lipoprotein a)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Lp-a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Lp-a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Lp-a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Lp-a. You can calculate the concentration of Rat Lp-a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
103-116
93-107
Average (%)
103
109
100
1:4
Range (%)
96-111
96-110
100-114
Average (%)
104
102
108
1:8
Range (%)
84-98
92-107
91-107
Average (%)
90
99
98
1:16
Range (%)
88-102
99-116
104-120
Average (%)
95
108
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.78
9.44
0.9
2.84
8.6
Standard deviation
0.12
0.29
0.88
0.11
0.34
0.88
C V (%)
12.24
10.43
9.32
12.22
11.97
10.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Lp-a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Lp-a in samples. No significant cross-reactivity or interference between Rat Lp-a and analogues was observed.
