The Rat LPTN (XCL1) Lymphotactin CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of LPTN (XCL1) levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for researchers in a variety of fields.LPTN (XCL1), also known as lymphotactin, is a chemokine protein that plays a crucial role in immune response regulation, cell recruitment, and inflammation. It is involved in various biological processes, including T cell migration and activation, making it a key target for studying immune system function and related diseases.
This CLIA kit is easy to use and provides rapid results, making it a convenient option for laboratories working on rat models and studying the role of LPTN (XCL1) in different physiological and pathological conditions. With its high sensitivity and specificity, the Rat LPTN (XCL1) Lymphotactin CLIA Kit is a valuable tool for advancing research in immunology, inflammation, and beyond.
Product Name:
Rat Lptn/XCL1 (Lymphotactin) CLIA Kit
SKU:
RTES00367
Target:
Rat Lptn/XCL1 (Lymphotactin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Lptn/XCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Lptn/XCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Lptn/XCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Lptn/XCL1. You can calculate the concentration of Rat Lptn/XCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
93-107
101-118
Average (%)
94
101
108
1:4
Range (%)
100-117
95-106
97-113
Average (%)
107
101
103
1:8
Range (%)
92-104
101-115
90-103
Average (%)
97
109
96
1:16
Range (%)
83-95
101-118
92-107
Average (%)
90
108
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.55
95.96
470.23
54.4
88.29
455.56
Standard deviation
5.68
8.57
32.59
5.85
6.97
30.07
C V (%)
10.61
8.93
6.93
10.75
7.89
6.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Lptn/XCL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Lptn/XCL1 in samples. No significant cross-reactivity or interference between Rat Lptn/XCL1 and analogues was observed.
