Rat MDC/CCL22 (Macrophage-Derived Chemokine) CLIA Kit
The Rat MDC (CCL22) Macrophage-Derived Chemokine CLIA Kit is a high-quality assay designed for the accurate quantification of CCL22 levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.CCL22, also known as macrophage-derived chemokine, plays a vital role in the immune response by attracting immune cells to sites of inflammation and infection.
Aberrant levels of CCL22 have been associated with various inflammatory conditions, making it a valuable biomarker for studying immune responses and developing potential therapeutic interventions.Overall, the Rat MDC (CCL22) Macrophage-Derived Chemokine CLIA Kit is a reliable tool for researchers looking to investigate the role of CCL22 in immune regulation and inflammatory diseases in rat models.
Product Name:
Rat MDC/CCL22 (Macrophage-Derived Chemokine) CLIA Kit
SKU:
RTES00375
Target:
Rat MDC/CCL22 (Macrophage-Derived Chemokine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MDC/CCL22. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MDC/CCL22 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MDC/CCL22, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MDC/CCL22. You can calculate the concentration of Rat MDC/CCL22 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
99-114
103-120
Average (%)
103
107
109
1:4
Range (%)
92-110
99-114
92-109
Average (%)
100
106
99
1:8
Range (%)
86-102
92-107
94-108
Average (%)
93
98
101
1:16
Range (%)
91-107
100-112
88-102
Average (%)
98
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.53
66.11
217.64
27.72
64.8
229.01
Standard deviation
2.93
5.19
17.32
2.42
5.82
18.98
C V (%)
11.04
7.85
7.96
8.73
8.98
8.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MDC/CCL22 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MDC/CCL22 in samples. No significant cross-reactivity or interference between Rat MDC/CCL22 and analogues was observed.
