Rat Microfibrillar Associated Protein 4 (MFAP4) ELISA Kit
The Rat Microfibrillar Associated Protein 4 (MFAP4) ELISA Kit is specifically designed for the accurate quantification of MFAP4 levels in rat serum, plasma, and tissue samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MFAP4 is a key protein associated with the regulation of microfibril assembly and tissue remodeling. Its role in fibrosis, cardiovascular diseases, and other pathologies make it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With the Rat MFAP4 ELISA Kit, researchers can confidently measure MFAP4 levels in rat samples, providing valuable insights into disease mechanisms and potential treatment strategies. Trust in this kit for precise and reliable results in your research endeavors.
Product Name:
Rat Microfibrillar Associated Protein 4 (MFAP4) ELISA Kit
Product Code:
RTDL00663
Size:
96 Assays
Target:
MFAP4
Synonyms:
Microfibril-Associated Glycoprotein 4
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
