Rat Microtubule Associated Protein Tau (MAPt) ELISA Kit
The Rat Microtubule-Associated Protein Tau (MAPT) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of MAPT levels in rat samples, including serum, plasma, and cell culture supernatants. This ELISA kit provides reliable and reproducible results, making it ideal for a variety of research applications.MAPT is a key protein involved in regulating microtubule stability and neuronal function. Dysregulation of MAPT has been implicated in neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease, making it an important biomarker for studying these conditions and potential therapeutic interventions.
With its high sensitivity and specificity, the Rat MAPT ELISA Kit offers researchers a valuable tool for quantifying MAPT levels and furthering our understanding of its role in neurodegenerative diseases.
Product Name:
Rat Microtubule Associated Protein Tau (MAPt) ELISA Kit
Product Code:
RTDL00651
Size:
96 Assays
Target:
MAPt
Synonyms:
DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, Neurofibrillary tangle protein, Tau Protein, Paired helical filament-tau, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
