Rat MIF (Macrophage Migration Inhibitory Factor) CLIA Kit
The Rat MIF (Macrophage Migration Inhibitory Factor) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MIF levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.MIF is a key cytokine involved in regulating the immune response and inflammatory processes. It plays a crucial role in various diseases such as autoimmune disorders, inflammatory conditions, and cancer.
Studying MIF levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Rat MIF CLIA Kit offers researchers a reliable and efficient solution for quantifying MIF levels in rat samples, enabling further exploration of MIF's role in disease pathology and treatment development.
Product Name:
Rat MIF (Macrophage Migration Inhibitory Factor) CLIA Kit
SKU:
RTES00374
Target:
Rat MIF (Macrophage Migration Inhibitory Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MIF. You can calculate the concentration of Rat MIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-117
95-107
93-106
Average (%)
107
102
99
1:4
Range (%)
87-101
95-109
95-106
Average (%)
92
103
101
1:8
Range (%)
91-106
102-119
87-100
Average (%)
99
109
92
1:16
Range (%)
90-106
94-110
99-115
Average (%)
98
100
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
99-110
105
Cell culture media (n=5)
96-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
3.05
6.98
1.13
3.03
7.18
Standard deviation
0.12
0.33
0.69
0.09
0.35
0.7
C V (%)
11.54
10.82
9.89
7.96
11.55
9.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MIF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MIF in samples. No significant cross-reactivity or interference between Rat MIF and analogues was observed.
