Rat MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) CLIA Kit
The Rat MIP-1 beta (Macrophage Inflammatory Protein-1 beta) CLIA Kit is a reliable and accurate assay designed for the detection of MIP-1 beta levels in rat samples such as serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it an essential tool for studying inflammatory processes in rat models.MIP-1 beta is a key chemokine involved in the recruitment and activation of macrophages and other immune cells, playing a crucial role in the regulation of inflammatory responses. Dysregulation of MIP-1 beta has been implicated in various diseases, including autoimmune disorders, infectious diseases, and inflammatory conditions, making it a valuable biomarker for understanding disease pathogenesis and developing therapeutic strategies.
The Rat MIP-1 beta CLIA Kit is easy to use and requires minimal sample volume, making it convenient for high-throughput screening and analysis. Whether you are studying immune responses, inflammation, or drug efficacy in rat models, this kit will provide the accurate and reliable data you need to advance your research. Visit the link provided for more information on the product.
Product Name:
Rat MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) CLIA Kit
SKU:
RTES00370
Target:
Rat MIP-1 beta (Macrophage Inflammatory Protein 1 Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MIP-1β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIP-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIP-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MIP-1β. You can calculate the concentration of Rat MIP-1β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
86-98
97-111
Average (%)
96
91
104
1:4
Range (%)
93-108
97-108
90-103
Average (%)
101
102
97
1:8
Range (%)
104-116
93-107
96-110
Average (%)
110
99
103
1:16
Range (%)
91-104
90-104
96-114
Average (%)
98
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
89-106
97
Cell culture media (n=5)
95-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.47
72.3
222.57
25.01
71.98
244.74
Standard deviation
2.41
5.13
17.96
2.92
6.23
23.79
C V (%)
9.85
7.1
8.07
11.68
8.66
9.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MIP-1β concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MIP-1β in samples. No significant cross-reactivity or interference between Rat MIP-1β and analogues was observed.
