The Rat MMP-10 (Matrix Metalloproteinase-10) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of MMP-10 levels in rat samples such as serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it an ideal tool for researchers studying the role of MMP-10 in various physiological and pathological processes.Matrix Metalloproteinase-10 is a key enzyme involved in extracellular matrix remodeling, cell migration, and tissue repair.
Dysregulation of MMP-10 has been implicated in various inflammatory diseases, cancer metastasis, and wound healing processes, highlighting its importance as a potential therapeutic target.With its ease of use and high performance, the Rat MMP-10 CLIA Kit from Assay Genie offers researchers a valuable tool for investigating the role of MMP-10 in health and disease, and ultimately, developing new strategies for diagnosis and treatment.
Product Name:
Rat MMP-10 (Matrix Metalloproteinase 10) CLIA Kit
SKU:
RTES00025
Target:
Rat MMP-10 (Matrix Metalloproteinase 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-10. You can calculate the concentration of Rat MMP-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
90-105
90-102
Average (%)
101
96
95
1:4
Range (%)
102-116
91-103
98-109
Average (%)
108
96
104
1:8
Range (%)
91-109
91-102
100-116
Average (%)
99
97
109
1:16
Range (%)
90-103
96-114
103-117
Average (%)
96
104
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.54
213.71
695.75
95.0
226.9
627.94
Standard deviation
10.34
18.61
58.1
11.56
24.62
46.22
C V (%)
10.28
8.71
8.35
12.17
10.85
7.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-10 in samples. No significant cross-reactivity or interference between Rat MMP-10 and analogues was observed.
