Based on the information provided in the URL, the Rat MMP-12 (Matrix Metalloproteinase 12) CLIA Kit is specifically tailored for the precise measurement of MMP-12 levels in rat samples. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MMP-12, also known as macrophage metalloelastase, plays a critical role in extracellular matrix remodeling and tissue repair. Dysregulation of MMP-12 is linked to various pathological conditions, including inflammatory diseases, pulmonary disorders, and cancer progression.
Therefore, this kit serves as a valuable tool for investigating the role of MMP-12 in disease pathogenesis and evaluating potential therapeutic interventions.Overall, the Rat MMP-12 CLIA Kit from Assay Genie is a reliable and versatile solution for researchers seeking to study the function and regulation of MMP-12 in rat models, facilitating advancements in the understanding and treatment of MMP-12-associated diseases.
Product Name:
Rat MMP-12 (Matrix Metalloproteinase 12) CLIA Kit
SKU:
RTES00026
Target:
Rat MMP-12 (Matrix Metalloproteinase 12)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-12. You can calculate the concentration of Rat MMP-12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
95-110
91-106
Average (%)
109
102
98
1:4
Range (%)
88-103
96-111
97-112
Average (%)
94
104
103
1:8
Range (%)
94-108
99-112
95-107
Average (%)
100
105
102
1:16
Range (%)
87-98
90-101
92-109
Average (%)
93
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-117
106
EDTA plasma (n=5)
102-115
107
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.03
116.65
411.82
47.83
125.05
437.47
Standard deviation
6.56
11.83
46.49
4.11
8.89
32.46
C V (%)
12.61
10.14
11.29
8.59
7.11
7.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-12 in samples. No significant cross-reactivity or interference between Rat MMP-12 and analogues was observed.
