The Rat MMP-17 (Matrix Metalloproteinase 17) CLIA Kit is a powerful tool for researchers looking to analyze MMP-17 levels in rat samples. This kit provides highly accurate and precise results, allowing for reliable data in a variety of research applications.Matrix Metalloproteinase 17 is an important enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a key role in processes such as wound healing, tissue repair, and inflammation. Dysregulation of MMP-17 has been linked to various diseases, including cancer, arthritis, and cardiovascular disorders, making it a valuable target for therapeutic interventions.
With its high sensitivity and specificity, the Rat MMP-17 CLIA Kit offers researchers a reliable method for measuring MMP-17 levels in biological samples. By studying the expression and activity of MMP-17, researchers can gain valuable insights into disease mechanisms and potential therapeutic strategies.
Product Name:
Rat MMP-17 (Matrix Metalloproteinase 17) CLIA Kit
SKU:
RTES00383
Target:
Rat MMP-17 (Matrix Metalloproteinase 17)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-17. You can calculate the concentration of Rat MMP-17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
91-103
91-101
Average (%)
98
98
96
1:4
Range (%)
86-98
87-103
94-104
Average (%)
91
94
99
1:8
Range (%)
88-101
87-100
95-105
Average (%)
94
93
100
1:16
Range (%)
91-103
97-111
96-109
Average (%)
96
104
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.63
172.66
723.55
109.67
176.2
737.2
Standard deviation
10.96
12.22
64.4
8.99
17.48
68.85
C V (%)
10.28
7.08
8.9
8.2
9.92
9.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-17 in samples. No significant cross-reactivity or interference between Rat MMP-17 and analogues was observed.
