The Rat MMP-24 (Matrix Metalloproteinase-24) CLIA Kit is a powerful tool for measuring MMP-24 levels in rat samples. This kit offers high sensitivity and accuracy, allowing for precise and reliable results for a variety of research applications.MMP-24, also known as membrane-type matrix metalloproteinase-24, is an important enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a key role in processes such as wound healing, tissue repair, and cancer metastasis, making it a valuable biomarker for studying these physiological and pathological pathways.
By using the Rat MMP-24 CLIA Kit, researchers can better understand the role of MMP-24 in various biological processes and diseases, paving the way for potential therapeutic interventions and targeted treatments. This kit is easy to use, with clear instructions and robust performance, making it a valuable asset for labs conducting cutting-edge research in the field of molecular biology.
Product Name:
Rat MMP-24 (Matrix Metalloproteinase 24) CLIA Kit
SKU:
RTES00384
Target:
Rat MMP-24 (Matrix Metalloproteinase 24)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-24. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-24, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-24. You can calculate the concentration of Rat MMP-24 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
87-100
97-112
Average (%)
95
94
103
1:4
Range (%)
91-105
85-100
89-100
Average (%)
98
92
94
1:8
Range (%)
87-99
94-111
102-118
Average (%)
94
101
109
1:16
Range (%)
100-114
88-101
96-111
Average (%)
107
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
95-109
100
Cell culture media (n=5)
99-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.61
240.7
684.58
105.67
244.26
722.76
Standard deviation
10.4
25.49
41.96
9.59
21.3
63.1
C V (%)
9.66
10.59
6.13
9.08
8.72
8.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-24 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-24 in samples. No significant cross-reactivity or interference between Rat MMP-24 and analogues was observed.
