The Rat MT1 (Metallothionein1) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of metallothionein1 levels in rat samples such as serum, plasma, and tissue homogenates. Metallothionein1 is a key protein involved in metal ion homeostasis and detoxification processes, making it a valuable biomarker for studying metal toxicity, oxidative stress, and other related conditions in rats.This CLIA kit offers reliable and reproducible results, allowing researchers to confidently analyze metallothionein1 levels in various experimental settings.
With its user-friendly protocol and excellent performance characteristics, this kit is well-suited for a wide range of research applications in the fields of toxicology, pharmacology, and environmental health. Unlock new insights into metal-associated pathologies and advance your research with the Rat MT1 (Metallothionein1) CLIA Kit from Assay Genie.
Product Name:
Rat MT1 (Metallothionein1) CLIA Kit
SKU:
RTES00391
Target:
Rat MT1 (Metallothionein1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MT1. You can calculate the concentration of Rat MT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
95-108
101-116
Average (%)
98
102
107
1:4
Range (%)
101-116
97-111
97-113
Average (%)
107
104
103
1:8
Range (%)
91-105
96-111
91-103
Average (%)
96
104
97
1:16
Range (%)
90-107
99-117
96-111
Average (%)
98
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.97
96.19
395.86
46.23
95.43
364.89
Standard deviation
3.85
10.62
42.16
3.86
7.06
30.58
C V (%)
8.96
11.04
10.65
8.35
7.4
8.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MT1 in samples. No significant cross-reactivity or interference between Rat MT1 and analogues was observed.
