The Rat MUC1 (Mucin 1) ELISA Kit is specifically designed for the precise and quantitative measurement of Mucin 1 levels in various rat biological samples.
Mucin 1 is a vital glycoprotein involved in cell hydration, protection, and cell signaling processes, making it an essential factor in cellular homeostasis and signaling pathways. With exceptional sensitivity and specificity, this ELISA kit from Assay Genie ensures reliable and reproducible results, enabling accurate analysis of Mucin 1 in your research samples. Manufactured under stringent quality control standards, the kit delivers robust performance and user-friendly procedures, offering researchers a reliable tool to investigate the roles of Mucin 1 in various biological processes and disease states. Trust in Assay Genie for high-quality ELISA kits that facilitate cutting-edge research in the field of Mucin 1 biology.
Product Name:
Rat MUC1 (Mucin 1) ELISA Kit
SKU:
AEES00477
Target:
Rat MUC1 (Mucin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MUC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MUC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MUC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MUC1. You can calculate the concentration of Rat MUC1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
97-114
89-102
Average (%)
90
104
94
1:4
Range (%)
87-99
83-97
82-93
Average (%)
93
90
88
1:8
Range (%)
88-98
83-96
86-96
Average (%)
93
89
91
1:16
Range (%)
89-101
81-93
87-99
Average (%)
96
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.33
4.12
0.5
1.24
4.23
Standard deviation
0.03
0.07
0.15
0.03
0.07
0.21
C V (%)
5.88
5.26
3.64
6.0
5.65
4.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MUC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MUC1 in samples. No significant cross-reactivity or interference between Rat MUC1 and analogues was observed.
