The Rat Neuroligin-1 (NLGN1) ELISA Kit from Assaygenie is a cutting-edge tool designed for the precise measurement of NLGN1 levels in rat serum, plasma, and tissue lysates. With its superior sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable asset for a variety of research applications.NLGN1 is a key protein involved in synaptic function and neurotransmission, playing a crucial role in neurodevelopmental disorders such as autism and schizophrenia.
By quantifying NLGN1 levels, researchers can gain valuable insights into synaptic communication and potential therapeutic targets for these conditions.Whether investigating neurological diseases, studying synaptic function, or exploring drug development, the Rat Neuroligin-1 (NLGN1) ELISA Kit provides the tools needed to advance scientific discoveries and pave the way for new treatment options. Choose Assaygenie's ELISA Kit for reliable and accurate measurements of NLGN1 levels in rat samples.
Product Name:
Rat Neuroligin 1 (NLGN1) ELISA Kit
Product Code:
RTDL00734
Size:
96 Assays
Target:
NLGN1
Synonyms:
Neuroligin I, Neuroligin-1
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
