The Rat Neurotensin (NT) ELISA Kit is a highly reliable and accurate tool for the quantitative detection of neurotensin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Neurotensin is a key neuropeptide that plays a crucial role in modulating various physiological functions, including neurotransmission, cardiovascular regulation, and gastrointestinal motility.
Dysregulation of neurotensin has been implicated in a range of neurological disorders, such as schizophrenia, Parkinson's disease, and Alzheimer's disease, making it a valuable biomarker for investigating these conditions and potential therapeutic interventions.Overall, the Rat Neurotensin (NT) ELISA Kit provides researchers with a powerful and accurate tool for studying the role of neurotensin in neurobiology research and drug development.
Product Name:
Rat Neurotensin (NT) ELISA Kit
Product Code:
RTDL00769
Size:
96 Assays
Target:
NT
Synonyms:
NTS, NTS1, NmN-125, NN, Tail peptide, Large neuromedin N
Detection Method:
Competition
Range:
4.687-300pmol/L
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
