The Rat NFIL3 CLIA Kit is a cutting-edge assay designed for the precise measurement of NFIL3 levels in rat samples, including serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable tool for various research applications.NFIL3, also known as E4BP4, is a key transcription factor that regulates gene expression and plays a critical role in various physiological processes.
Its dysregulation has been linked to a range of diseases, including autoimmune disorders, inflammatory conditions, and cancer. By detecting and quantifying NFIL3 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Trust the Rat NFIL3 CLIA Kit to provide reliable data for your research needs, advancing our understanding of NFIL3 biology and its implications for human health.
Product Name:
Rat NFIL-3 CLIA Kit
SKU:
RTES00414
Target:
Rat NFIL-3
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NFIL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFIL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFIL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NFIL3. You can calculate the concentration of Rat NFIL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
93-109
99-115
Average (%)
110
101
105
1:4
Range (%)
90-104
102-117
93-106
Average (%)
98
108
100
1:8
Range (%)
101-115
98-109
96-109
Average (%)
107
103
102
1:16
Range (%)
102-120
94-107
86-101
Average (%)
109
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.47
258.99
984.87
108.97
278.11
992.46
Standard deviation
13.27
27.19
104.49
12.35
28.12
86.34
C V (%)
12.23
10.5
10.61
11.33
10.11
8.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NFIL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NFIL3 in samples. No significant cross-reactivity or interference between Rat NFIL3 and analogues was observed.
