Rat NFKB-p105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
The Rat NFKB-p105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit is meticulously crafted for the precise quantitative determination of NFKB-p105 levels in a variety of rat biological samples.
NFKB-p105, also known as NF-kappa-B1 precursor, is a critical subunit of the nuclear factor-kappa B (NF-κB) complex, playing a pivotal role in regulating gene expression involved in immune responses, inflammation, cell survival, and various disease processes. Its dynamic functions make it a prominent target in deciphering the molecular mechanisms underlying inflammatory pathways, immune disorders, and pathogenic conditions. By accurately measuring NFKB-p105 levels in rat samples, researchers can gain valuable insights into the intricate signaling cascades controlled by the NF-κB complex, thereby unraveling its involvement in health, disease progression, and potential therapeutic strategies. Understanding the nuances of NFKB-p105 expression can pave the way for innovative research and therapeutic developments aimed at modulating NF-κB-mediated pathways for beneficial outcomes in various pathological conditions. The Rat NFKB-p105 ELISA Kit by Assay Genie offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. With its robust performance and user-friendly protocols, this kit is an excellent choice for research endeavors seeking to delve deep into the complexity of NF-κB signaling and its implications in health and disease.
Product Name:
Rat NFKB-p105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
SKU:
AEES00497
Target:
Rat NFKB-p105 (Nuclear factor NF-kappa-B p105 subunit)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFKB-p105. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFKB-p105 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFKB-p105, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFKB-p105. You can calculate the concentration of Rat NFKB-p105 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
97-113
94-108
Average (%)
90
105
101
1:4
Range (%)
88-104
82-93
87-98
Average (%)
95
87
93
1:8
Range (%)
91-104
86-100
87-102
Average (%)
98
92
94
1:16
Range (%)
92-108
81-94
83-94
Average (%)
100
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.42
4.64
0.51
1.36
4.23
Standard deviation
0.03
0.06
0.21
0.03
0.07
0.17
C V (%)
6.38
4.23
4.53
5.88
5.15
4.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p105 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NFKB-p105 in samples. No significant cross-reactivity or interference between Rat NFKB-p105 and analogues was observed.
