Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit
The Rat NGAL (Neutrophil Gelatinase-Associated Lipocalin) CLIA Kit is specifically designed for the quantitative measurement of NGAL levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.NGAL is a protein that is associated with inflammatory responses and kidney damage, making it a valuable biomarker for studying conditions such as acute kidney injury, sepsis, and certain types of cancers.
By using this CLIA kit, researchers can gain valuable insights into disease mechanisms and potential therapeutic strategies.Overall, the Rat NGAL CLIA Kit is a powerful tool for studying the role of NGAL in various physiological and pathological processes, offering researchers a reliable method for quantifying NGAL levels in rat samples.
Product Name:
Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit
SKU:
RTES00408
Target:
Rat NGAL (Neutrophil Gelatinase Associated Lipocalin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NGAL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NGAL. You can calculate the concentration of Rat NGAL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
94-104
88-100
Average (%)
105
99
94
1:4
Range (%)
87-101
92-105
90-105
Average (%)
94
98
97
1:8
Range (%)
101-115
100-113
103-117
Average (%)
107
107
110
1:16
Range (%)
87-102
89-103
91-105
Average (%)
93
94
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
90-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.07
94.14
493.27
56.91
101.01
541.12
Standard deviation
4.77
8.63
31.27
6.43
10.8
43.24
C V (%)
8.82
9.17
6.34
11.3
10.69
7.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NGAL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NGAL in samples. No significant cross-reactivity or interference between Rat NGAL and analogues was observed.
