Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit
The Rat NOS1 (NNOS) Nitric Oxide Synthase 1 Neuronal CLIA Kit is a cutting-edge solution designed for precise and efficient detection of NOS1 levels in rat samples. This innovative kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.NOS1, also known as neuronal nitric oxide synthase, is a key enzyme involved in the synthesis of nitric oxide, a signaling molecule that plays a crucial role in various physiological processes. Dysregulation of NOS1 has been linked to numerous health conditions, including neurodegenerative diseases, cardiovascular disorders, and inflammatory conditions.
Therefore, the Rat NOS1 CLIA Kit serves as a valuable tool for studying the role of NOS1 in these diseases and developing potential therapeutic interventions.Don't miss out on the opportunity to elevate your research with the Rat NOS1 Nitric Oxide Synthase 1 Neuronal CLIA Kit from Assay Genie. Order yours today and unlock new insights into the intricate mechanisms underlying nitric oxide signaling in rat models!
Product Name:
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit
SKU:
RTES00632
Target:
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS1/nNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NOS1/nNOS. You can calculate the concentration of Rat NOS1/nNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
88-101
95-106
Average (%)
101
93
100
1:4
Range (%)
88-102
86-99
90-104
Average (%)
96
94
97
1:8
Range (%)
90-105
94-107
99-111
Average (%)
97
100
105
1:16
Range (%)
96-107
98-111
94-109
Average (%)
101
104
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.2
307.05
752.28
95.17
326.18
819.78
Standard deviation
12.07
30.09
61.31
9.53
30.01
77.39
C V (%)
12.42
9.8
8.15
10.01
9.2
9.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NOS1/nNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NOS1/nNOS in samples. No significant cross-reactivity or interference between Rat NOS1/nNOS and analogues was observed.
