The Rat NPHN (Nephrin) ELISA Kit is specifically designed for the precise measurement of nephrin levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for various research purposes.Nephrin is a key protein that plays a vital role in maintaining the structural integrity of the kidney's filtration barrier.
Dysregulation of nephrin has been linked to several kidney diseases, including nephrotic syndrome and diabetic nephropathy. Therefore, this ELISA kit is essential for studying these conditions and developing potential therapeutic interventions.Order the Rat NPHN (Nephrin) ELISA Kit today from Assay Genie and advance your research in the field of kidney diseases.
Product Name:
Rat NPHN (Nephrin) ELISA Kit
SKU:
RTES01034
Target:
Rat NPHN (Nephrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NPHN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NPHN. You can calculate the concentration of Rat NPHN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
97-115
93-108
Average (%)
98
105
99
1:4
Range (%)
95-110
87-101
83-96
Average (%)
100
93
88
1:8
Range (%)
88-101
86-100
84-96
Average (%)
95
91
90
1:16
Range (%)
86-102
82-97
87-103
Average (%)
93
88
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.27
4.27
0.53
1.29
3.89
Standard deviation
0.03
0.06
0.19
0.03
0.07
0.14
C V (%)
5.56
4.72
4.45
5.66
5.43
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NPHN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NPHN in samples. No significant cross-reactivity or interference between Rat NPHN and analogues was observed.
