The Rat NRP2 (Neuropilin 2) ELISA Kit is a cutting-edge tool designed to accurately quantify the levels of Neuropilin 2 in various rat biological samples. Neuropilin 2 is a multifunctional type I transmembrane receptor with diverse roles in axonal guidance, angiogenesis, and immune regulation. Its interactions with semaphorins and vascular endothelial growth factors (VEGFs) contribute significantly to neural development, angiogenesis, and immune response modulation. This ELISA kit empowers researchers to precisely measure and analyze the expression of Neuropilin 2, facilitating a deeper understanding of its intricate functions in normal physiological processes and pathological conditions.
By elucidating the signaling pathways associated with Neuropilin 2, researchers can explore its involvement in neural development, vascular biology, and immunomodulation, paving the way for potential therapeutic advancements and biomedical applications. The Rat NRP2 ELISA Kit offers remarkable sensitivity and specificity, ensuring accurate and reproducible results in the laboratory. Manufactured with stringent quality control measures, this kit guarantees robust performance and user-friendly protocols, making it an indispensable tool for scientific research aimed at unraveling the complexities of Neuropilin 2 biology in rat models.
Product Name:
Rat NRP2 (Neuropilin 2) ELISA Kit
SKU:
AEES00560
Target:
Rat NRP2 (Neuropilin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NRP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NRP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NRP2. You can calculate the concentration of Rat NRP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-103
93-107
89-103
Average (%)
98
98
94
1:4
Range (%)
92-107
93-105
90-103
Average (%)
99
99
95
1:8
Range (%)
93-104
92-108
90-104
Average (%)
99
100
95
1:16
Range (%)
94-108
89-102
90-100
Average (%)
99
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
96-110
101
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
264.67
452.85
2360.88
270.4
444.5
2147.55
Standard deviation
16.28
22.96
97.5
18.17
20.85
92.77
C V (%)
6.15
5.07
4.13
6.72
4.69
4.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NRP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NRP2 in samples. No significant cross-reactivity or interference between Rat NRP2 and analogues was observed.
