Description
Rat NT-3 (Neurotrophin 3) ELISA Kit
The Rat NT-3 (Neurotrophin 3) ELISA Kit is a precise assay specifically designed for the quantitative detection of Neurotrophin 3 levels in various rat biological samples. NT-3 is a crucial member of the neurotrophin family, playing a pivotal role in the growth, maintenance, and survival of neurons in the central and peripheral nervous systems. As a neurotrophic factor, NT-3 is involved in neurogenesis and synaptic plasticity, contributing to the development and function of the nervous system. This ELISA kit serves as a valuable tool for researchers investigating the effects of NT-3 on neuronal growth, regeneration, and repair mechanisms.
By accurately quantifying NT-3 levels in rat samples, researchers can gain insights into neurodevelopmental processes, neural plasticity, and potential therapeutic strategies for neurodegenerative conditions. The Rat NT-3 ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit offers robust performance and user-friendly protocols, making it an ideal choice for research studies aiming to elucidate the intricate roles of Neurotrophin 3 in neural development and function.
| Product Name: | Rat NT-3 (Neurotrophin 3) ELISA Kit |
| Product Code: | AEES00495 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.10 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat NT-3 in samples. No significant cross-reactivity or interference between Rat NT-3 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat NT-3 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NT-3. You can calculate the concentration of Rat NT-3 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NT-3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NT-3 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.49 | 1.00 | 4.60 | 0.46 | 0.91 | 4.99 |
| Standard deviation | 0.03 | 0.04 | 0.17 | 0.03 | 0.05 | 0.15 |
| CV (%) | 6.12 | 4.00 | 3.70 | 6.52 | 5.49 | 3.01 |
Recovery
The recovery of Rat NT-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-96 | 91 |
| EDTA plasma (n=8) | 92-104 | 98 |
| Cell culture media (n=8) | 95-107 | 101 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
