The Rat NT-4 (Neurotrophin-4) CLIA Kit is a top-of-the-line assay designed for precise and reliable measurement of neurotrophin-4 levels in rat samples. This kit boasts high sensitivity and specificity, providing accurate and reproducible results for various research applications.Neurotrophin-4 is a key neurotrophic factor that plays a crucial role in the development and maintenance of the nervous system. It is involved in neuronal survival, differentiation, and synaptic plasticity, making it a valuable target for studying neurological disorders and potential therapeutic interventions.
Whether you are investigating neurodevelopmental processes, neurodegenerative diseases, or exploring potential treatments, the Rat NT-4 CLIA Kit is an essential tool for your research needs. Trust in its performance and reliability to advance your scientific discoveries.
Product Name:
Rat NT-4 (Neurotrophin 4) CLIA Kit
SKU:
RTES00405
Target:
Rat NT-4 (Neurotrophin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NT-4. You can calculate the concentration of Rat NT-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
93-109
87-101
Average (%)
91
100
94
1:4
Range (%)
99-112
94-109
86-100
Average (%)
104
102
92
1:8
Range (%)
85-97
86-101
90-105
Average (%)
90
92
97
1:16
Range (%)
96-111
95-108
91-101
Average (%)
104
100
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
97
EDTA plasma (n=5)
100-117
108
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.85
115.92
275.45
36.65
120.48
273.15
Standard deviation
3.11
8.91
30.88
4.37
8.93
21.85
C V (%)
8.44
7.69
11.21
11.92
7.41
8.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NT-4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NT-4 in samples. No significant cross-reactivity or interference between Rat NT-4 and analogues was observed.
