Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide) CLIA Kit
The Rat NT-Pro-ANP (N-Terminal Pro Atrial Natriuretic Peptide) CLIA Kit is specifically designed for the precise measurement of NT-Pro-ANP levels in rat serum, plasma, and urine samples. This cutting-edge kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.NT-Pro-ANP is a key biomarker associated with heart function and cardiac health, playing a vital role in regulating blood pressure and fluid balance.
Changes in NT-Pro-ANP levels can indicate conditions such as heart failure, hypertension, and renal dysfunction, making it an invaluable tool for studying these cardiovascular disorders and developing potential treatment strategies.Overall, the Rat NT-Pro-ANP CLIA Kit is a valuable resource for researchers and clinicians seeking to delve deeper into the intricacies of cardiovascular physiology and pathology, paving the way for advancements in cardiac care and management.
Product Name:
Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide) CLIA Kit
SKU:
RTES00625
Target:
Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proANP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proANP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NT-proANP. You can calculate the concentration of Rat NT-proANP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
87-99
101-118
Average (%)
104
92
107
1:4
Range (%)
86-100
90-104
94-106
Average (%)
92
97
99
1:8
Range (%)
85-97
88-102
97-114
Average (%)
91
95
105
1:16
Range (%)
87-100
90-101
96-112
Average (%)
94
95
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
108
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
195.01
436.68
1726.45
182.44
445.63
1852.04
Standard deviation
24.86
36.72
146.23
23.28
47.19
190.39
C V (%)
12.75
8.41
8.47
12.76
10.59
10.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NT-proANP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NT-proANP in samples. No significant cross-reactivity or interference between Rat NT-proANP and analogues was observed.
