Rat Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) ELISA Kit
The Rat NR1D2 (Nuclear Receptor Subfamily 1 Group D, Member 2) ELISA Kit is a powerful tool for the precise measurement of NR1D2 levels in rat samples such as serum, plasma, and tissue homogenates. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.NR1D2 is a key member of the nuclear receptor subfamily, playing a crucial role in regulating circadian rhythms and metabolic processes. Dysregulation of NR1D2 has been implicated in various diseases, including metabolic disorders, cardiovascular diseases, and neurological conditions, making it a valuable biomarker for investigating these pathologies and developing potential therapeutic interventions.
Overall, the Rat NR1D2 ELISA Kit provides researchers with a reliable tool to study the role of NR1D2 in health and disease, offering valuable insights into its molecular mechanisms and potential therapeutic applications.
Product Name:
Rat Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
