The Rat OPG (Osteoprotegerin) ELISA Kit is an advanced assay system that enables precise and quantitative analysis of Osteoprotegerin levels in a wide range of biological samples obtained from rat models. Osteoprotegerin, a key regulatory protein in bone metabolism and cardiovascular health, serves as a critical modulator of bone resorption, vascular calcification, and inflammation. By accurately measuring Osteoprotegerin concentrations, this ELISA kit offers valuable insights into bone homeostasis, vascular health, and inflammatory processes in rat subjects. Accurate quantification of Osteoprotegerin levels is essential for studying the intricate interplay between bone biology, cardiovascular function, and inflammatory responses.
The Rat OPG ELISA Kit empowers researchers to explore the role of Osteoprotegerin in bone remodeling, vascular health, and inflammatory diseases, providing a deeper understanding of the underlying mechanisms and potential therapeutic targets. Crafted with a focus on accuracy and reliability, the Rat OPG ELISA Kit delivers exceptional sensitivity and specificity, ensuring consistent and reproducible results. Researchers can rely on this kit to generate reliable data, facilitating in-depth investigations into the physiological and pathological functions of Osteoprotegerin in rat models.
Product Name:
Rat OPG (Osteoprotegerin) ELISA Kit
SKU:
AEES00521
Target:
Rat OPG (Osteoprotegerin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OPG. You can calculate the concentration of Rat OPG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-111
92-101
85-99
Average (%)
105
97
91
1:4
Range (%)
97-112
83-97
93-100
Average (%)
105
91
97
1:8
Range (%)
90-96
97-108
84-95
Average (%)
93
103
91
1:16
Range (%)
96-103
87-98
85-98
Average (%)
99
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
90-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.96
124.69
487.62
44.44
104.83
417.2
Standard deviation
2.56
6.0
26.62
2.29
4.73
17.31
C V (%)
5.57
4.81
5.46
5.15
4.51
4.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat OPG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OPG in samples. No significant cross-reactivity or interference between Rat OPG and analogues was observed.
