The Rat Orexin (OX) ELISA Kit is a top-quality assay kit developed for the precise measurement of orexin levels in rat samples, including serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit provides accurate and reproducible results, making it an invaluable tool for a wide range of research studies.Orexin, also known as hypocretin, is a neuropeptide that plays a key role in regulating sleep and wakefulness, appetite, and energy balance. Dysregulation of orexin signaling has been implicated in various disorders, including sleep disorders, obesity, and neurological conditions.
Therefore, monitoring orexin levels can provide valuable insights into these conditions and aid in the development of novel therapeutic strategies.Whether investigating the role of orexin in physiological processes or exploring its potential as a therapeutic target, the Rat Orexin (OX) ELISA Kit is the ideal choice for researchers seeking reliable and accurate quantification of orexin levels in rat samples. Trust Assay Genie for high-quality products that deliver exceptional results in your research endeavors.
Product Name:
Rat Orexin (OX) ELISA Kit
Product Code:
RTDL00791
Size:
96 Assays
Target:
OX
Synonyms:
HCRT, PPOX, PPORX, Hypocretin
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
