Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit
The Rat PAI-1 (Plasminogen Activator Inhibitor-1) CLIA Kit is a powerful tool for accurately measuring levels of PAI-1 in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results that are perfect for various research purposes.PAI-1 is a vital protein that regulates the body's fibrinolysis process, playing a critical role in blood clotting and cardiovascular health. Abnormal levels of PAI-1 have been linked to various diseases, including thrombosis, atherosclerosis, and metabolic disorders, making it a valuable biomarker for understanding these conditions and developing potential treatments.
With the Rat PAI-1 CLIA Kit, researchers can confidently study the role of PAI-1 in disease progression and therapeutic interventions, paving the way for new discoveries and advancements in the field of biomedical research.
Product Name:
Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit
SKU:
RTES00442
Target:
Rat PAI-1 (Plasminogen Activator Inhibitor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PAI-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PAI-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PAI-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PAI-1. You can calculate the concentration of Rat PAI-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
97-111
93-106
Average (%)
101
105
99
1:4
Range (%)
85-99
92-105
99-116
Average (%)
90
98
106
1:8
Range (%)
97-114
86-100
101-118
Average (%)
105
93
109
1:16
Range (%)
90-103
88-102
99-114
Average (%)
96
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
102
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.05
466.61
1710.0
195.09
481.94
1692.65
Standard deviation
25.0
47.22
141.25
22.63
43.57
141.67
C V (%)
12.25
10.12
8.26
11.6
9.04
8.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PAI-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PAI-1 in samples. No significant cross-reactivity or interference between Rat PAI-1 and analogues was observed.
