Rat PAI-2 (Plasminogen Activator Inhibitor 2) CLIA Kit
The Rat PAI-2 (Plasminogen Activator Inhibitor-2) CLIA Kit is a reliable and accurate tool for measuring PAI-2 levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PAI-2 is a key regulator of the fibrinolytic system, inhibiting plasminogen activators and playing a critical role in processes such as blood clotting and wound healing.
Dysregulation of PAI-2 has been implicated in various diseases and conditions, highlighting its importance as a biomarker for understanding and potentially treating these disorders.Overall, the Rat PAI-2 CLIA Kit provides researchers with a valuable tool for studying the role of PAI-2 in physiological and pathological processes in rat models, paving the way for new insights and therapeutic interventions.
Product Name:
Rat PAI-2 (Plasminogen Activator Inhibitor 2) CLIA Kit
SKU:
RTES00443
Target:
Rat PAI-2 (Plasminogen Activator Inhibitor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PAI-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PAI-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PAI-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PAI-2. You can calculate the concentration of Rat PAI-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
102-120
101-117
Average (%)
96
109
109
1:4
Range (%)
94-107
86-97
103-117
Average (%)
99
92
109
1:8
Range (%)
88-102
94-109
87-104
Average (%)
93
102
95
1:16
Range (%)
90-106
85-100
86-100
Average (%)
96
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.74
8.59
0.86
2.5
9.37
Standard deviation
0.08
0.3
0.83
0.08
0.21
0.97
C V (%)
8.7
10.95
9.66
9.3
8.4
10.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PAI-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PAI-2 in samples. No significant cross-reactivity or interference between Rat PAI-2 and analogues was observed.
