The Rat PC4 (Positive Cofactor 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PC4 levels in rat samples such as serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.PC4, also known as positive cofactor 4, is a transcriptional coactivator that plays a critical role in gene regulation and transcriptional activation. It is involved in various cellular processes, including DNA repair, cell cycle regulation, and development.
Dysregulation of PC4 has been linked to numerous diseases, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.Overall, the Rat PC4 CLIA Kit offers researchers a powerful tool for investigating the role of PC4 in different biological processes and disease states, leading to a better understanding of its functions and implications in health and disease.
Product Name:
Rat PC4 (Positive Cofactor 4) CLIA Kit
SKU:
RTES00460
Target:
Rat PC4 (Positive Cofactor 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PC4. You can calculate the concentration of Rat PC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
103-120
100-114
Average (%)
91
110
107
1:4
Range (%)
92-108
87-102
87-99
Average (%)
100
94
94
1:8
Range (%)
96-111
84-97
99-113
Average (%)
103
90
107
1:16
Range (%)
100-116
99-117
100-116
Average (%)
107
106
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
103-119
108
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.32
87.61
289.38
38.87
91.14
307.38
Standard deviation
3.8
7.7
28.76
4.33
10.58
28.4
C V (%)
10.18
8.79
9.94
11.14
11.61
9.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PC4 in samples. No significant cross-reactivity or interference between Rat PC4 and analogues was observed.
