Rat PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit
The Rat PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit is a powerful tool for measuring levels of PCNA in rat samples. With high sensitivity and specificity, this kit provides accurate and reproducible results for researchers studying cell proliferation and DNA replication in rats.PCNA is a key protein involved in DNA synthesis and repair, serving as a marker for actively dividing cells. Its levels can indicate the rate of cell proliferation and are important for understanding processes such as embryogenesis, tissue regeneration, and tumorigenesis.
This kit is suitable for use with rat serum, plasma, and tissue homogenates, offering versatility for various experimental setups. With its ease of use and reliability, the Rat PCNA CLIA Kit is an essential tool for investigating cell cycle dynamics and potential therapeutic targets in rat models.
Product Name:
Rat PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit
SKU:
RTES00472
Target:
Rat PCNA (Proliferating Cell Nuclear Antigen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PCNA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCNA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PCNA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PCNA. You can calculate the concentration of Rat PCNA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
83-97
93-107
Average (%)
95
90
99
1:4
Range (%)
96-112
97-113
96-107
Average (%)
103
103
102
1:8
Range (%)
90-103
91-102
93-108
Average (%)
97
97
101
1:16
Range (%)
96-113
84-96
88-101
Average (%)
104
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
99
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.12
194.96
724.41
90.17
208.15
665.47
Standard deviation
11.97
19.81
61.21
11.48
23.5
55.37
C V (%)
12.32
10.16
8.45
12.73
11.29
8.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PCNA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PCNA in samples. No significant cross-reactivity or interference between Rat PCNA and analogues was observed.
