Rat PDGFB (Platelet Derived Growth Factor Subunit B) CLIA Kit
The Rat PDGFB (Platelet-Derived Growth Factor Subunit B) CLIA Kit is designed for the accurate measurement of PDGFB levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PDGFB is a key protein involved in cell growth and division, playing a critical role in various physiological processes such as wound healing, tissue repair, and vascular remodeling.
Abnormal PDGFB expression has been implicated in diseases like cancer, fibrosis, and cardiovascular disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.With the Rat PDGFB CLIA Kit, researchers can gain valuable insights into the role of PDGFB in health and disease, paving the way for advancements in biomedical research and drug discovery.
Product Name:
Rat PDGFB (Platelet Derived Growth Factor Subunit B) CLIA Kit
SKU:
RTES00449
Target:
Rat PDGFB (Platelet Derived Growth Factor Subunit B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PDGF-B. You can calculate the concentration of Rat PDGF-B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
95-112
96-109
Average (%)
100
103
103
1:4
Range (%)
92-107
88-101
94-107
Average (%)
99
93
99
1:8
Range (%)
103-115
87-100
85-96
Average (%)
109
93
90
1:16
Range (%)
102-115
87-99
90-105
Average (%)
108
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.42
82.57
383.97
38.51
87.49
388.9
Standard deviation
3.92
9.48
31.83
4.18
9.41
24.0
C V (%)
10.48
11.48
8.29
10.85
10.76
6.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PDGF-B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGF-B in samples. No significant cross-reactivity or interference between Rat PDGF-B and analogues was observed.
