The Rat PDGFR-alpha (Platelet-Derived Growth Factor Soluble Receptor Alpha) CLIA Kit is an innovative assay designed for the precise measurement of PDGFR-alpha levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PDGFR-alpha is a key receptor involved in cellular signaling and growth, playing a crucial role in processes such as cell proliferation and tissue repair. Dysregulation of PDGFR-alpha has been associated with various diseases, including cancer and fibrotic disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and reliable performance, the Rat PDGFR-alpha CLIA Kit is a valuable tool for researchers investigating the role of PDGFR-alpha in health and disease. Its ease of use and robust design make it suitable for a wide range of experimental settings, providing precise and dependable results every time.
Rat PDGFsR- alpha (Platelet-Derived Growth Factor Soluble Receptor alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGFsR-α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGFsR-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGFsR-α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PDGFsR-α. You can calculate the concentration of Rat PDGFsR-α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
93-107
93-105
Average (%)
107
100
99
1:4
Range (%)
98-113
97-111
94-106
Average (%)
106
104
100
1:8
Range (%)
103-115
98-112
96-113
Average (%)
109
104
103
1:16
Range (%)
93-107
100-115
91-105
Average (%)
100
107
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.68
223.5
911.69
99.81
213.59
976.46
Standard deviation
10.98
19.33
64.73
9.57
22.77
98.72
C V (%)
10.49
8.65
7.1
9.59
10.66
10.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PDGFsR-α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGFsR-α in samples. No significant cross-reactivity or interference between Rat PDGFsR-α and analogues was observed.
