The Rat PGR (Progesterone Receptor) CLIA Kit is specifically designed for the precise measurement of progesterone receptor levels in rat samples. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.The progesterone receptor is a key protein involved in hormone signaling and plays a critical role in reproductive processes and female health. Dysregulation of the progesterone receptor has been linked to conditions such as infertility, menstrual disorders, and certain cancers, highlighting its importance as a biomarker for studying and treating these conditions.
Overall, the Rat PGR CLIA Kit provides researchers with a reliable tool for investigating progesterone receptor levels in rats and understanding its implications in various physiological and pathological processes.
Product Name:
Rat PGR (Progesterone Receptor) CLIA Kit
SKU:
RTES00466
Target:
Rat PGR (Progesterone Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PGR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PGR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PGR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PGR. You can calculate the concentration of Rat PGR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-120
85-101
85-99
Average (%)
110
92
91
1:4
Range (%)
87-99
96-112
95-106
Average (%)
94
102
100
1:8
Range (%)
96-108
95-112
99-115
Average (%)
102
103
106
1:16
Range (%)
94-110
98-110
89-105
Average (%)
101
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.48
585.45
1667.08
216.07
603.88
1555.98
Standard deviation
20.9
50.29
127.7
27.38
61.96
150.77
C V (%)
10.22
8.59
7.66
12.67
10.26
9.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PGR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PGR in samples. No significant cross-reactivity or interference between Rat PGR and analogues was observed.
