Rat Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit
The Rat Phosphodiesterase 3B (cGMP-inhibited PDE3B) ELISA Kit is a reliable tool for measuring levels of PDE3B in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Phosphodiesterase 3B is an enzyme that plays a critical role in regulating cGMP levels and signaling pathways in cells. Dysregulation of PDE3B has been linked to various diseases, including metabolic disorders, cardiovascular diseases, and diabetes.
By measuring PDE3B levels, researchers can gain valuable insights into the mechanisms underlying these conditions and explore potential therapeutic targets.Overall, the Rat Phosphodiesterase 3B (cGMP-inhibited PDE3B) ELISA Kit is a valuable tool for studying PDE3B-related pathways and diseases in rat models, offering researchers a reliable and accurate method for quantifying PDE3B levels in biological samples.
Product Name:
Rat Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit
Product Code:
RTDL00810
Size:
96 Assays
Target:
PDE3B
Synonyms:
HcGIP1, cGIPDE1, CGIPDE1, CGIP1, CGI-PDE B, Cyclic GMP-inhibited phosphodiesterase B
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
