Based on the information from the URL provided, the Rat Pi Proinsulin CLIA Kit (RTES00469) is specifically designed for the quantitative measurement of proinsulin levels in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for research studies focusing on diabetes, metabolism, and insulin regulation in rats.Proinsulin is a precursor to insulin that plays a crucial role in glucose metabolism and the regulation of blood sugar levels. Abnormal levels of proinsulin can indicate insulin resistance, impaired insulin production, or other metabolic disorders, making it a significant biomarker for studying diabetes and related conditions.
Overall, the Rat Pi Proinsulin CLIA Kit is an essential tool for researchers looking to better understand the mechanisms of insulin regulation and explore potential therapeutic interventions for metabolic disorders in rat models. With its reliable performance and ease of use, this kit is a valuable asset for advancing research in the field of diabetes and metabolism.
Product Name:
Rat PI (Proinsulin) CLIA Kit
SKU:
RTES00469
Target:
Rat PI (Proinsulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PI. You can calculate the concentration of Rat PI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
99-113
97-112
Average (%)
101
106
102
1:4
Range (%)
88-100
84-95
88-103
Average (%)
93
90
95
1:8
Range (%)
97-110
86-97
96-113
Average (%)
104
91
104
1:16
Range (%)
93-105
98-115
98-111
Average (%)
99
106
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
103
EDTA plasma (n=5)
95-112
102
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.89
598.56
1505.13
197.04
612.84
1369.8
Standard deviation
18.34
53.03
111.98
20.0
48.48
156.16
C V (%)
9.61
8.86
7.44
10.15
7.91
11.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PI in samples. No significant cross-reactivity or interference between Rat PI and analogues was observed.
