The Rat PKR1 (Prokineticin Receptor 1) CLIA Kit is a reliable and sensitive assay designed for the accurate detection of PKR1 levels in rat serum, plasma, and cell culture supernatants. This kit ensures consistent and reproducible results, making it an ideal tool for research applications in the field of neuroscience and endocrinology.PKR1 is a key receptor involved in various physiological processes, including circadian rhythm regulation, neurogenesis, and pain modulation.
Dysregulation of PKR1 has been implicated in conditions such as depression, insomnia, and chronic pain, highlighting its importance as a potential therapeutic target and biomarker for these disorders.The Rat PKR1 CLIA Kit offers researchers a versatile and reliable solution for studying the role of PKR1 in various biological processes and disease states, facilitating the development of novel therapeutic interventions and personalized treatment strategies.
Product Name:
Rat PKR1 (Prokineticin Receptor 1) CLIA Kit
SKU:
RTES00471
Target:
Rat PKR1 (Prokineticin Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PKR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PKR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PKR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PKR1. You can calculate the concentration of Rat PKR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
84-97
100-111
Average (%)
101
91
106
1:4
Range (%)
98-112
87-102
88-100
Average (%)
106
93
95
1:8
Range (%)
92-108
83-96
96-112
Average (%)
99
90
102
1:16
Range (%)
100-115
100-113
94-108
Average (%)
108
106
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.51
279.05
755.17
98.11
270.15
770.49
Standard deviation
7.76
29.24
46.37
10.57
29.55
76.74
C V (%)
8.12
10.48
6.14
10.77
10.94
9.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PKR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PKR1 in samples. No significant cross-reactivity or interference between Rat PKR1 and analogues was observed.
