Rat PLAU/uPA (Urokinase-Type Plasminogen Activator) CLIA Kit
The Rat PLAU (UPA) Urokinase Type Plasminogen Activator CLIA Kit is specifically designed for the accurate detection of urokinase-type plasminogen activator levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Urokinase-type plasminogen activator is a key enzyme involved in the regulation of fibrinolysis and extracellular matrix remodeling.
It plays a crucial role in processes such as wound healing, tissue remodeling, and cancer metastasis, making it a valuable biomarker for studying these physiological and pathological conditions.Researchers and scientists can use the Rat PLAU Urokinase Type Plasminogen Activator CLIA Kit to accurately measure urokinase-type plasminogen activator levels in rat samples, enabling further investigation into its biological functions and potential therapeutic applications.
Product Name:
Rat PLAU/uPA (Urokinase-Type Plasminogen Activator) CLIA Kit
SKU:
RTES00585
Target:
Rat PLAU/uPA (Urokinase-Type Plasminogen Activator)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PLAU/uPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PLAU/uPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PLAU/uPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PLAU/uPA. You can calculate the concentration of Rat PLAU/uPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
98-112
87-100
Average (%)
98
104
94
1:4
Range (%)
98-112
88-102
92-103
Average (%)
105
94
97
1:8
Range (%)
87-100
84-97
97-110
Average (%)
92
91
102
1:16
Range (%)
85-97
87-100
89-104
Average (%)
92
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
102-116
108
Cell culture media (n=5)
95-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.45
52.97
179.97
29.44
57.36
167.17
Standard deviation
3.52
6.09
16.07
2.63
4.49
17.08
C V (%)
12.82
11.5
8.93
8.93
7.83
10.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PLAU/uPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PLAU/uPA in samples. No significant cross-reactivity or interference between Rat PLAU/uPA and analogues was observed.
