Rat PPAR-alpha (Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit
The Rat PPAR-? (Peroxisome Proliferator-Activated Receptor Alpha) ELISA Kit is specifically designed to quantitatively measure the levels of Peroxisome Proliferator-Activated Receptor Alpha in rat biological samples. PPAR-? is a nuclear receptor that plays a crucial role in regulating genes involved in fatty acid oxidation and energy homeostasis. It serves as a key modulator of lipid metabolism, particularly in response to fasting and nutrient availability, and contributes to the maintenance of metabolic balance and energy production. Accurate assessment of PPAR-? levels using this ELISA kit provides valuable insights into the molecular pathways governing fatty acid catabolism, mitochondrial biogenesis, and lipid utilization. Researchers can utilize this kit to investigate the impact of PPAR-? on metabolic processes, including the regulation of cholesterol levels and ketogenesis.
Understanding the intricate role of PPAR-? in energy metabolism is essential for unraveling its implications in metabolic disorders, such as dyslipidemia, obesity, and metabolic syndrome. The Rat PPAR-? ELISA Kit from Assay Genie ensures high sensitivity and specificity, allowing for reliable and reproducible results. With stringent quality control measures in place, this kit delivers excellent performance and ease of use, making it a valuable tool for research studies focused on elucidating the molecular mechanisms governed by PPAR-? in metabolic regulation and metabolic-related diseases.
Product Name:
Rat PPAR-alpha (Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit
Product Code:
AEES00506
Assay Type:
Sandwich
Format:
96T
Assay Time:
3.5h
Reactivity:
Rat
Detection Range:
0.31-20 ng/mL
Sensitivity:
0.19 ng/mL
Sample Type & Sample Volume:
Serum, plasma and other biological fluids, 100?L
Specificity:
This kit recognizes Rat PPAR-? in samples. No significant cross-reactivity or interference between Rat PPAR-? and analogues was observed.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PPAR-? concentrations in serum, plasma and other biological fluids.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-?. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PPAR-? and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PPAR-?, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PPAR-?. You can calculate the concentration of Rat PPAR-? in the samples by comparing the OD of the samples to the standard curve.
Kit Components:
An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells x 12 strips
-20?, 6 months
Reference Standard
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 120 ?L
Concentrated HRP Conjugate (100×)
1 vial, 120 ?L
-20?(Protect from light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8?(Protect from light)
Stop Solution
1 vial, 10 mL
2-8?
Plate Sealer
5 pieces
Manual
1 copy
Certificate of Analysis
1 copy
1.
Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C
2.
Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3.
Aspirate and wash the plate for 3 times
4.
Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5.
Add 90?L Substrate Reagent. Incubate for 15 min at 37°C
6.
Add 50?L Stop Solution
7.
Read the plate at 450nm immediately. Calculation of the results
Amadi, P.U., et al.
Dual intestinal-hepatic modulation by phenolic acids improves metabolic-dysfunction associated steatohepatitis
