The Rat PRF1 (Perforin 1) CLIA Kit is a highly sensitive assay designed for the precise quantification of perforin-1 levels in rat samples. This kit is ideal for use with serum, plasma, and cell culture supernatants, providing accurate and reliable results for a variety of research applications.Perforin-1 is a key protein involved in the immune system, specifically in the process of cell-mediated cytotoxicity. It plays a crucial role in the elimination of virus-infected cells and cancer cells by forming pores in the target cell membrane.
Monitoring perforin-1 levels can provide valuable insights into immune function and response to various diseases, making it a valuable tool for research in immunology and oncology.With its advanced technology and ease of use, the Rat PRF1 CLIA Kit from AssayGenie is a valuable resource for researchers studying immune responses, cancer biology, and related fields. Get reliable and reproducible results with this high-quality assay kit.
Product Name:
Rat PRF1 (Perforin 1) CLIA Kit
SKU:
RTES00431
Target:
Rat PRF1 (Perforin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PRF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PRF1. You can calculate the concentration of Rat PRF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
91-108
93-108
Average (%)
97
98
100
1:4
Range (%)
98-110
101-115
95-110
Average (%)
104
108
102
1:8
Range (%)
89-100
93-104
101-115
Average (%)
95
99
109
1:16
Range (%)
100-113
87-103
87-101
Average (%)
107
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
84-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
215.91
619.45
1357.54
209.82
567.98
1404.47
Standard deviation
22.8
58.79
95.3
25.6
64.64
109.55
C V (%)
10.56
9.49
7.02
12.2
11.38
7.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PRF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PRF1 in samples. No significant cross-reactivity or interference between Rat PRF1 and analogues was observed.
