Rat PRO-ANP (Pro Atrial Natriuretic Peptide) CLIA Kit
The Rat Pro-ANP (Pro-Atrial Natriuretic Peptide) CLIA Kit is a cutting-edge assay designed for the precise measurement of Pro-ANP levels in rat samples. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Pro-ANP is a key biomarker involved in cardiovascular health, specifically in regulating blood pressure and fluid balance.
Dysregulation of Pro-ANP levels has been linked to conditions such as heart failure and hypertension, making it a valuable marker for studying these diseases and developing targeted therapies.With its advanced technology and reliable performance, the Rat Pro-ANP CLIA Kit is an indispensable tool for researchers seeking to delve deeper into the role of Pro-ANP in cardiovascular physiology and pathology.
Product Name:
Rat PRO-ANP (Pro Atrial Natriuretic Peptide) CLIA Kit
SKU:
RTES00462
Target:
Rat PRO-ANP (Pro Atrial Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Pro-ANP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Pro-ANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Pro-ANP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Pro-ANP. You can calculate the concentration of Rat Pro-ANP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-116
98-112
94-105
Average (%)
110
104
99
1:4
Range (%)
92-107
93-104
92-108
Average (%)
99
99
99
1:8
Range (%)
96-111
91-105
91-103
Average (%)
102
99
96
1:16
Range (%)
100-112
94-111
87-102
Average (%)
106
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.83
52.68
184.52
27.75
50.57
189.2
Standard deviation
3.42
6.05
12.03
3.36
5.58
15.17
C V (%)
12.75
11.48
6.52
12.11
11.03
8.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Pro-ANP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Pro-ANP in samples. No significant cross-reactivity or interference between Rat Pro-ANP and analogues was observed.
