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Rat Progesterone PharmaGenie ELISA Kit (SBRS0151)

SKU:
SBRS0151
Product type:
ELISA Kit
Reactivity:
Rat
Application:
ELISA
ELISA Type:
Competitive
€649
Frequently bought together:

Description

Rat Progesterone PharmaGenie ELISA - Kit Information

Progesterone also known as P4 is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens, and is the major naturally occurring human progestogen.

Rat Progesterone PharmaGenie ELISA - Kit Data

Product SKU:SBRS0151
Size:96T
Application:This ELISA kit is designed to detect human, mouse, and rat Progesterone.
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 ng/ml
Sensitivity:2.1 ng/ml
Recommended Dilution:Human: 8X, Mouse: 8X, Rat: 8X
Storage/Stability:Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Rat Progesterone PharmaGenie ELISA Kit (SBES0151) - Product Images

Product ImageStandard Curve for Rat Progesterone PharmaGenie ELISA Kit (SBES0151)

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
6.HRP-Streptavidin
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Rat Progesterone ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed.
2.Add 100 ul detection antibody to each well.
3.Incubate 1.5 h at RT or O/N at 4°C.
4.Add 100 ul standard or sample to each well.
5.Incubate 2.5 h at RT.
6.Add 100 ul prepared streptavidin solution.
7.Incubate 45 min at RT.
8.Add 100 ul TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul Stop Solution to each well.
11.Read plate at 450 nm immediately.