Description
Rat Progesterone PharmaGenie ELISA - Kit Information
Progesterone also known as P4 is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens, and is the major naturally occurring human progestogen.
Rat Progesterone PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS0151 |
| Size: | 96T |
| Application: | This ELISA kit is designed to detect human, mouse, and rat Progesterone. |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 ng/ml |
| Sensitivity: | 2.1 ng/ml |
| Recommended Dilution: | Human: 8X, Mouse: 8X, Rat: 8X |
| Storage/Stability: | Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
Rat Progesterone PharmaGenie ELISA Kit (SBES0151) - Product Images
 | Standard Curve for Rat Progesterone PharmaGenie ELISA Kit (SBES0151) |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Standard Peptide |
| 4. | Assay Diluent(s) |
| 5. | Biotinylated Peptide |
| 6. | HRP-Streptavidin |
| 7. | TMB One-Step Substrate |
| 8. | Stop Solution |
| 9. | Assay Diagram |
| 10. | Positive Control Sample |
| 11. | Capture Antibody |
| 12. | Technical Manual |
Other materials and equipment required:
The Assay Genie Rat Progesterone ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Kit Protocol:
| 1. | Prepare all reagents, samples and standards as instructed. |
| 2. | Add 100 ul detection antibody to each well. |
| 3. | Incubate 1.5 h at RT or O/N at 4°C. |
| 4. | Add 100 ul standard or sample to each well. |
| 5. | Incubate 2.5 h at RT. |
| 6. | Add 100 ul prepared streptavidin solution. |
| 7. | Incubate 45 min at RT. |
| 8. | Add 100 ul TMB One-Step Substrate Reagent to each well. |
| 9. | Incubate 30 min at RT. |
| 10. | Add 50 ul Stop Solution to each well. |
| 11. | Read plate at 450 nm immediately. |
