Rat Protease Activated Receptor 4 (PAR4) ELISA Kit
The Rat Protease-Activated Receptor 4 (PAR4) ELISA Kit is a reliable and accurate tool for the quantitative detection of PAR4 levels in rat samples. This kit is specially designed to provide high sensitivity and specificity for precise and reproducible results, making it suitable for a variety of research applications.PAR4 is a key protein involved in the activation of platelets and regulation of thrombosis, making it essential for understanding blood clotting mechanisms and related disorders.
Its role in cardiovascular diseases and other pathological conditions makes it a valuable biomarker for research and potential therapeutic interventions.With the Rat PAR4 ELISA Kit, researchers can confidently study the function and expression of PAR4 in rat models, advancing their understanding of thrombosis and related diseases. Get accurate and reliable results with this high-quality ELISA kit from Assay Genie.
Product Name:
Rat Protease Activated Receptor 4 (PAR4) ELISA Kit
Product Code:
RTDL00799
Size:
96 Assays
Target:
PAR4
Synonyms:
F2RL3, PAR-4, Coagulation Factor II Thrombin Receptor-Like 3, Coagulation factor II receptor-like 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
