The Rat Protein Kinase C Epsilon (PKCε) ELISA Kit is a reliable and accurate tool for detecting levels of PKCε in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for various research applications.Protein kinase C epsilon (PKCε) is a key enzyme involved in intracellular signaling pathways, playing a crucial role in cell growth, differentiation, and survival. Dysregulation of PKCε has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions, highlighting its significance as a potential therapeutic target.
By measuring PKCε levels, researchers can gain valuable insights into the molecular mechanisms underlying disease development and progression, paving the way for the development of targeted therapies. The Rat Protein Kinase C Epsilon (PKCε) ELISA Kit is an essential tool for studying PKCε function in a preclinical setting.
Product Name:
Rat Protein Kinase C Epsilon (PKCe) ELISA Kit
Product Code:
RTDL00840
Size:
96 Assays
Target:
PKCe
Synonyms:
PKC-E, PRKCE, PRKC-E, NPKC-Epsilon
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
