Rat PTHR2 (Parathyroid Hormone Receptor 2) CLIA Kit
The Rat PTHR2 (Parathyroid Hormone Receptor 2) CLIA Kit is a specialized assay designed for the accurate measurement of PTHR2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.PTHR2 is a key receptor involved in the regulation of parathyroid hormone, playing a crucial role in calcium homeostasis and bone development.
Dysregulation of PTHR2 has been linked to bone disorders such as osteoporosis and hyperparathyroidism, making it an important target for studying these conditions and developing potential treatment strategies.Overall, the Rat PTHR2 CLIA Kit is a valuable tool for researchers interested in studying the role of PTHR2 in bone metabolism and related diseases, providing accurate and consistent results to advance scientific understanding in this field.
Product Name:
Rat PTHR2 (Parathyroid Hormone Receptor 2) CLIA Kit
SKU:
RTES00427
Target:
Rat PTHR2 (Parathyroid Hormone Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PTHR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTHR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTHR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PTHR2. You can calculate the concentration of Rat PTHR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
86-100
100-117
Average (%)
104
92
107
1:4
Range (%)
90-102
93-110
99-115
Average (%)
97
100
105
1:8
Range (%)
95-108
101-116
92-107
Average (%)
101
110
97
1:16
Range (%)
97-109
93-108
90-105
Average (%)
103
98
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.38
88.75
359.54
50.71
83.77
379.02
Standard deviation
3.92
8.69
35.59
6.56
6.55
32.63
C V (%)
8.1
9.79
9.9
12.94
7.82
8.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PTHR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PTHR2 in samples. No significant cross-reactivity or interference between Rat PTHR2 and analogues was observed.
