Rat PUMA (P53 Upregulated Modulator of Apoptosis) CLIA Kit
The Rat PUMA (p53 Upregulated Modulator of Apoptosis) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PUMA levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for researchers studying apoptosis and cell death pathways.PUMA is a key regulator of apoptosis, playing a critical role in controlling cell survival and death. Dysregulation of PUMA has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
By measuring PUMA levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic interventions.Overall, the Rat PUMA CLIA Kit offers a valuable tool for studying apoptosis and its role in disease pathogenesis, providing researchers with the means to advance their understanding of cell death processes.
Product Name:
Rat PUMA (P53 Upregulated Modulator of Apoptosis) CLIA Kit
SKU:
RTES00426
Target:
Rat PUMA (P53 Upregulated Modulator of Apoptosis)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PUMA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PUMA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PUMA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PUMA. You can calculate the concentration of Rat PUMA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
98-115
92-106
Average (%)
93
106
97
1:4
Range (%)
98-114
100-116
97-113
Average (%)
104
107
103
1:8
Range (%)
89-102
97-113
104-117
Average (%)
96
103
110
1:16
Range (%)
93-105
95-107
94-110
Average (%)
99
102
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
107
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
99-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.47
208.3
997.38
106.78
198.78
1084.13
Standard deviation
11.34
21.2
67.42
11.31
21.23
96.27
C V (%)
11.18
10.18
6.76
10.59
10.68
8.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PUMA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PUMA in samples. No significant cross-reactivity or interference between Rat PUMA and analogues was observed.
