The Rat PYY (Peptide YY) CLIA Kit is a cutting-edge diagnostic tool designed for the precise and reliable measurement of PYY levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering accurate and reproducible results that are essential for various research applications.PYY, also known as Peptide YY, is a critical hormone involved in regulating appetite and energy metabolism. It plays a vital role in controlling food intake and body weight, making it a valuable biomarker for studying obesity, diabetes, and other metabolic disorders.
By accurately measuring PYY levels, researchers can gain insights into the mechanisms underlying these conditions and develop novel therapeutic strategies.Overall, the Rat PYY CLIA Kit is a powerful tool for advancing research in the fields of metabolism, endocrinology, and obesity, providing researchers with the means to explore the intricate pathways involved in appetite regulation and energy balance.
Product Name:
Rat PYY (Peptide YY) CLIA Kit
SKU:
RTES00430
Target:
Rat PYY (Peptide YY)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PYY. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PYY. You can calculate the concentration of Rat PYY in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
89-101
100-111
Average (%)
94
95
105
1:4
Range (%)
96-112
95-110
95-108
Average (%)
104
102
103
1:8
Range (%)
100-115
89-102
97-110
Average (%)
108
94
103
1:16
Range (%)
101-119
103-117
86-99
Average (%)
109
110
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
103-114
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.95
58.47
248.46
25.66
62.62
263.35
Standard deviation
2.4
6.33
22.11
2.37
4.48
19.51
C V (%)
10.02
10.83
8.9
9.24
7.15
7.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PYY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PYY in samples. No significant cross-reactivity or interference between Rat PYY and analogues was observed.
