Rat RANk (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit
The Rat Rank (Receptor Activator of Nuclear Factor Kappa-B) CLIA Kit is a reliable and accurate assay designed for the quantitative measurement of Rank levels in rat serum, plasma, and tissue homogenate samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. Rank is a key protein involved in the regulation of bone metabolism and immune response. It plays a crucial role in bone remodeling, osteoclast differentiation, and inflammatory processes, making it a valuable biomarker for studying skeletal disorders, autoimmune diseases, and cancer metastasis.
With its easy-to-use format and quick assay time, the Rat Rank CLIA Kit is an essential tool for researchers looking to explore the role of Rank in various biological processes and disease mechanisms. Order now to advance your research and uncover new insights into Rank biology.
Product Name:
Rat RANk (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit
SKU:
RTES00090
Target:
Rat RANk (Receptor Activator of Nuclear Factor Kappa B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RANκ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RANκ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RANκ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat RANκ. You can calculate the concentration of Rat RANκ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
89-105
88-102
Average (%)
92
97
93
1:4
Range (%)
85-97
88-100
89-104
Average (%)
91
93
95
1:8
Range (%)
87-99
92-107
92-106
Average (%)
92
98
99
1:16
Range (%)
102-118
89-104
87-98
Average (%)
108
96
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
92-108
98
Cell culture media (n=5)
97-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.37
186.04
892.11
104.72
188.76
855.4
Standard deviation
10.5
14.25
69.23
11.25
20.25
63.81
C V (%)
9.87
7.66
7.76
10.74
10.73
7.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat RANκ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RANκ in samples. No significant cross-reactivity or interference between Rat RANκ and analogues was observed.
