Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit
The Rat RANKL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of RANKL levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.RANKL is a key regulator of bone metabolism and plays a crucial role in osteoclast differentiation, activation, and survival. Dysregulation of RANKL signaling is associated with various bone-related disorders such as osteoporosis and bone metastasis.
Therefore, measuring RANKL levels can provide valuable insights into bone health and disease progression.By using the Rat RANKL CLIA Kit, researchers can accurately quantify RANKL levels in rat samples, allowing for a better understanding of the mechanisms underlying bone remodeling and skeletal homeostasis. This kit is an essential tool for investigating the role of RANKL in bone disorders and developing potential therapeutic interventions.
Product Name:
Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit
SKU:
RTES00498
Target:
Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RANκL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat RANκL. You can calculate the concentration of Rat RANκL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
103-115
101-115
Average (%)
95
109
109
1:4
Range (%)
90-103
96-111
92-106
Average (%)
96
104
98
1:8
Range (%)
87-99
88-102
88-102
Average (%)
94
93
94
1:16
Range (%)
96-111
100-113
88-101
Average (%)
103
106
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.51
63.94
240.21
22.32
62.4
251.19
Standard deviation
2.4
5.79
19.17
2.48
7.31
26.83
C V (%)
10.21
9.06
7.98
11.11
11.71
10.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat RANκL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RANκL in samples. No significant cross-reactivity or interference between Rat RANκL and analogues was observed.
