Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit
The Rat RANKL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit is a powerful tool for the accurate measurement of RANKL levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, delivering precise and consistent results for a variety of research applications.RANKL is a key regulator of bone metabolism, playing a vital role in osteoclast differentiation and activation. Abnormal RANKL expression has been linked to conditions such as osteoporosis, arthritis, and bone metastasis, making it a valuable marker for investigating these diseases and developing potential interventions.
With its advanced technology and user-friendly design, the Rat RANKL ELISA Kit from Assay Genie provides researchers with a reliable tool for studying RANKL signaling pathways and exploring therapeutic strategies for bone-related disorders. Get accurate and reproducible results with this cutting-edge kit.
Product Name:
Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit
SKU:
RTES00706
Target:
Rat RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat RANκL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat RANκL. You can calculate the concentration of Rat RANκL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
91-105
94-107
Average (%)
102
98
99
1:4
Range (%)
98-112
86-100
94-109
Average (%)
105
93
101
1:8
Range (%)
96-110
84-96
91-107
Average (%)
103
90
99
1:16
Range (%)
96-110
85-101
94-107
Average (%)
102
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
95-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.8
148.1
396.9
46.4
141.2
378.0
Standard deviation
3.1
5.9
19.4
2.5
5.9
15.5
C V (%)
6.77
3.98
4.89
5.39
4.18
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat RANκL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RANκL in samples. No significant cross-reactivity or interference between Rat RANκL and analogues was observed.
